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Quantitative proteomics analysis of Salmonella-infected mouse cecum

ABSTRACT: The ECM is a complex fibrillar network of macromolecules that provides structural and mechanical support to the intestinal tissue. One abundant component of the ECM observed in the Salmonella-driven intestinal edema is the mechanosensitive glycoprotein fibronectin. Combining mechanosensitive staining for relaxed fibronectin with total fibronectin revealed that fibronectin fibers present in the edema are in a tensed molecular conformation. Co-staining with fibrin(ogen) indicates a provisional matrix, similar to what is observed in response to skin injury. The absence of low tensional fibronectin fibers and the additional finding of a high number of protease inhibitors in the edema proteome could indicate a critical role of stretched fibronectin fibers in maintaining tissue integrity in the severely inflamed cecum. Understanding these processes may provide valuable functional diagnostic markers of intestinal disease progression.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Salmonella Enterica Subsp. Enterica Mus Musculus (mouse)

TISSUE(S): Cecum

SUBMITTER: Ronja Rappold

LAB HEAD: Emma Slack

PROVIDER: PXD043256 | Pride | 2024-09-16

REPOSITORIES: Pride

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	20220628_MK_EVO_KK_FAIMS_1CV_Whisper100_20SPD_1475_HRMS1DIA_60k_Ronja_880M2.raw	Raw
	20220628_MK_EVO_KK_FAIMS_1CV_Whisper100_20SPD_1475_HRMS1DIA_60k_Ronja_982M2.raw	Raw
	20220629_MK_EVO_KK_FAIMS_1CV_Whisper100_20SPD_1475_HRMS1DIA_60k_Ronja_873M.raw	Raw
	20220629_MK_EVO_KK_FAIMS_1CV_Whisper100_20SPD_1475_HRMS1DIA_60k_Ronja_873ME.raw	Raw
	20220629_MK_EVO_KK_FAIMS_1CV_Whisper100_20SPD_1475_HRMS1DIA_60k_Ronja_875ME.raw	Raw

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