Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

56 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MRNA-seq of MCF10A USP22 knockdown cells

ABSTRACT: This analysis identifies genes which are affected in MCF10A cells upon siRNA-mediated USP22 knockdown

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Steven Johnsen

PROVIDER: E-MTAB-8247 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

mRNA-seq of SW480 USP22 knockdown cells

Project description:This analysis identifies genes which are affected in SW480 cells upon siRNA-mediated USP22 knockdown

2019-12-05 | E-MTAB-8214 | biostudies-arrayexpress

mRNA-seq of SW837 USP22 knockdown cells

Project description:This analysis identifies genes which are affected in SW837 cells upon siRNA-mediated USP22 knockdown

2019-12-06 | E-MTAB-8215 | biostudies-arrayexpress

mRNA-seq of HCT116 USP22 knockdown cells

Project description:This analysis identifies genes which are affected in HCT116 cells upon USP22 knockdown

2019-10-26 | E-MTAB-7393 | biostudies-arrayexpress

mRNA-seq of HCC1954 siControl and siUSP22

Project description:The analysis of genes affected upon USP22 kcnockdown in HCC1954 cells

2020-08-31 | E-MTAB-8256 | biostudies-arrayexpress

mRNA-seq of HCC1806 cells, treated with siControl or siRNF40 for 48 hours

Project description:The analysis identifies genes affected by RNF40 silencing in HCC1806 cells

2023-07-20 | E-MTAB-11860 | biostudies-arrayexpress

mRNA-seq of HCT116 RNF40 knockdown cells upon TNF-alpha stimulation

Project description:This analysis identifies genes which are affected upon RNF40 knockdown cells and/or TNF-alpha stimulation

2018-10-22 | E-MTAB-7197 | biostudies-arrayexpress

USP22 supports the aggressive behavior of basal-like breast cancer by stimulating cellular respiration

Project description:Breast cancer (BC), the most frequent tumor entity in women globally, shows a high therapeutic response in early and non-metastatic stages. However, triple-negative BC (TNBC), enriched with cancer stem cells (CSCs), presents significant challenges due to its chemoresistant and metastatic nature. Ubiquitin Specific Proteinase 22 (USP22) has emerged as a key player in promoting CSC functions, contributing to resistance to conventional therapies, tumor relapse, metastasis, and poor survival across various cancers, including BC. The specific role of USP22 in TNBC, however, remains underexplored. In this study, we employed the MMTV-cre, Usp22fl/fl transgenic mouse model to investigate USP22's influence on stem cell-like properties in mammary tissue. High-throughput transcriptomic analyses, combined with publicly available patient data and TNBC culture models, were utilized to elucidate USP22's role in CSC characteristics of TNBC. Our findings reveal that USP22 enhances CSC properties and drug tolerance by supporting oxidative phosphorylation, a key factor in the poor response to conventional therapies in aggressive BC subtypes. The study uncovers a novel tumor-supportive role of USP22 in sustaining cellular respiration, which contributes to the drug-tolerant behavior of HER2+-BC and TNBC cells. This highlights USP22 as a potential therapeutic target, offering new avenues to optimize standard treatments and address the aggressiveness of these malignancies.

2023-12-31 | E-MTAB-13577 | biostudies-arrayexpress

RNF20/RNF40 supports aggressive behavior in cervical cancer by regulating peroxisome-based anti-ferroptotic mechanism

Project description:Cervical cancer treatments sometimes prove ineffective, indicating a need for personalized therapies. The present study demonstrate a strong correlation between high levels of the RNF20/RNF40 protein complex and aggressive cervical cancer. Our transcriptome analyzes uncovered a previously unknown role of this protein complex regulating peroxisomal pathway genes, which are crucial for lipid metabolism and the balance of reactive oxygen species. The loss of RNF20/RNF40 leads to reduced effectiveness of these genes, increasing lipid peroxidation and inducing a form of programmed cell death known as ferroptosis. These findings suggest that targeting the RNF20/RNF40 protein complex and its regulation capabilities could lead to new treatments for aggressive cervical cancer.

2025-03-09 | E-MTAB-13917 | biostudies-arrayexpress

RNA-seq on HER2-driven murine mammary carcinomas (MMTV-Erbb2; MMTV-cre) without or with a cre-mediated homozygous deletion of Usp22 (MMTV-Erbb2; MMTV-cre; Usp22 fl/fl)

Project description:The analysis identifies genes which are dependent on USP22 expression in murine HER2-driven murine mammary carcinomas.

2021-05-26 | E-MTAB-9331 | biostudies-arrayexpress

mRNA-seq of HCC1954 siControl and siRNF40

Project description:The analysis of genes affected upon RNF40 knockdown in HCC1954 cells

2020-11-16 | E-MTAB-9234 | biostudies-arrayexpress