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RNA-Seq of monoclonal cell lines after CRISPR/Cas9-mediated editing of box C/D snoRNA genes (SNORDs)

ABSTRACT: In this study monoclonal cell lines carrying mutations in snoRNA genes (SNORD74, SNORD75, SNORD77, SNORD80) were obtained. Next, whole transcriptome analysis of obtained cell lines was performed on an Illumina NextSeq 500 platform. RNA-seq libraries was constructed on PolyA mRNA-enriched fraction. The comparison of the expression levels of genes and transcripts in RNA-Seq experiments was carried out using CuffDiff tool. Mutations in SNORD75 in the obtained monoclonal cell line were shown to result in aberrant splicing of Gas5.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Anastasiya Matveeva

PROVIDER: E-MTAB-8269 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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