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RNA-seq of splenic B cells derived from WT and Itch KO mice

ABSTRACT: We found that the E3 ubiquitin ligase Itch significantly affects early B-cell differentiation. To explore the role of Itch in late B-cell differentiation, we sorted B cells from WT and Itch KO mice. To explore the effect of Itch deficency on gene expression, we determined mRNA profiles in B cells from WT and Itch KO mice by RNA-seq. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.

INSTRUMENT(S): Agilent 2100 Bioanalyzer, Illumina HiSeq 4000, Ribo-Zero Gold (Illumina)

ORGANISM(S): Mus musculus

SUBMITTER: Renxi Wang

PROVIDER: E-MTAB-8273 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-seq of splenic B cells derived from WT and Itch cKO mice

Project description:We found that the E3 ubiquitin ligase Itch significantly affects early B-cell differentiation. To explore the role of Itch in late B-cell differentiation, we sorted B cells from WT and Itch cKO mice. To explore the effect of Itch deficency on gene expression, we determined mRNA profiles in B cells from WT and Itch cKO mice by RNA-seq. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.

2020-03-01 | E-MTAB-8281 | biostudies-arrayexpress

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Single cell RNA-seq and VDJ-seq of B cells from sheep red blood cells-primed WT and Stch cKO mice

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Orientation-specific RAG activity in chromosomal loop domain contribute to Tcrd V(D)J recombination during T cell development

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