Single-cell RNA-sequencing of OMP-GFP mouse olfactory sensory neurons
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ABSTRACT: We sequenced the transcriptome of 34 single olfactory sensory neurons (OSNs) manually picked from OMP-GFP mice, ensuring the cells expressed high levels of GFP, a marker of mature OSNs. We pooled the olfactory mucosa from 2 male and 2 female animals, to obtain single-cell suspensions for cell picking. We only picked healthy-looking cells. From each, we constructed libraries for single-cell RNA-sequencing, to characterise the diversity of OR genes expressed.
INSTRUMENT(S): HiSeq X Ten
ORGANISM(S): Mus musculus
SUBMITTER: Zhen Zeng
PROVIDER: E-MTAB-8285 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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