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Regulatory T and T helper cells from BXD mouse strains

ABSTRACT: Parental and BXD mouse lines were received from Jackson Laboratory and The Oak Ridge National Laboratory. Splenocytes were isolated and stained with anti-CD4 and anti-CD25 antibodies. CD4+ T cells were separated into CD4+CD25+ Treg and CD4+CD25- Th cells. Tregs and Th cell were collected from spleens of 31 BXD recombinant inbred strains and of the parental mouse strains DBA/2J and C57BL/6J. Gene expression was measured by microarrays. The comparative analysis of the transcriptomes from the two cell populations allowed us to identify many novel differentially expressed genes. Furthermore, the analysis of cis- and trans-expression Quantitative Trail Loci (eQTLs) showed that both common and unique regulatory mechanisms are active in the two cell types.

ORGANISM(S): Mus musculus

SUBMITTER: Claudia Pommerenke 

PROVIDER: E-MTAB-836 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Expression QTL mapping in regulatory and helper T cells from the BXD family of strains reveals novel cell-specific genes, gene-gene interactions and candidate genes for auto-immune disease.

Alberts Rudi R   Chen Hairong H   Pommerenke Claudia C   Smit August B AB   Spijker Sabine S   Williams Robert W RW   Geffers Robert R   Bruder Dunja D   Schughart Klaus K  

BMC genomics 20111219

<h4>Background</h4>Regulatory T cells (Tregs) play an essential role in the control of the immune response. Treg cells represent important targets for therapeutic interventions of the immune system. Therefore, it will be very important to understand in more detail which genes are specifically activated in Treg cells versus T helper (Th) cells, and which gene regulatory circuits may be involved in specifying and maintaining Treg cell homeostasis.<h4>Results</h4>We isolated Treg and Th cells from  ...[more]

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