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Unknown,Transcriptomics,Genomics,Proteomics

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The retroelement Lx9 puts a brake on the immune response to virus infection

ABSTRACT: The mammalian genome has an abundance of transposable elements, but understanding their contribution to complex biological systems is poorly understood. Here, we report the CRISPR/Cas9 deletion of a retrotransposon (Lx9c11) in mice and its effect on the immune response to virus infection. The regulatory role for Lx9c11 was assessed by RNA-seq analysis of knockout and wild-type mice, treated with CVB4 virus. Long read nanopore RNA-seq of transcripts enriched from Slfn1 locus (mm10, chr11:83109157-83116657).

INSTRUMENT(S): PromethION, Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Nenad Bartonicek

PROVIDER: E-MTAB-8436 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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