Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Single-cell gene expression of germinal center B cells from CXCL12-conditionally deficient and –sufficient mice


ABSTRACT: The chemokines CXCL13 and CXCL12 are reported to be important for the germinal center reaction. Since CXCL12-deficient mice are embryonically lethal, here we took advantage of the Cxcl13-Cre/TdTomato mouse models to genetically ablate CXCL12 from B cell-interacting reticular cells and examine the molecular consequence on germinal center B cells. Spatial segregation of follicular dendritic cells, germinal center B cells and follicular helper T cells is impaired in Cxcl13-Cre/TdTomato Cxcl12fl/fl mice. Single cell transcriptomic analysis revealed that all germinal center B cell subsets (corresponding to distinct stages of the germinal center response) are present in draining lymph nodes of immunized CXCL12-conditionally deficient mice. While most transcriptional regulators of the germinal center response are unperturbed by the genetic perturbation of CXCL12, Bach2 levels were elevated in germinal center B cells from lymph nodes of Cxcl12fl/fl mice. Moreover, single cell B cell receptor sequencing revealed that germinal center B cells in Cxcl13-Cre/TdTomato Cxcl12fl/fl mice harbour a lower mutational burden when compared to germinal center B cells isolated from immunized control mice. Gene expression profiles were validated by flow cytometry and suggest that the provision of CXCL12 by reticular cells governs efficient germinal center responses.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Mechthild Lütge 

PROVIDER: E-MTAB-8454 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2020-05-12 | E-MTAB-8445 | biostudies-arrayexpress
2020-05-12 | E-MTAB-8904 | biostudies-arrayexpress
2012-06-13 | E-GEOD-38696 | biostudies-arrayexpress
2012-06-13 | E-GEOD-38712 | biostudies-arrayexpress
2010-09-07 | E-GEOD-23925 | biostudies-arrayexpress
2023-07-25 | E-MTAB-12499 | biostudies-arrayexpress
2023-07-25 | E-MTAB-12638 | biostudies-arrayexpress
2023-03-30 | E-MTAB-11738 | biostudies-arrayexpress
2010-04-28 | E-GEOD-21380 | biostudies-arrayexpress
2021-06-01 | E-MTAB-10056 | biostudies-arrayexpress