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Germinal Center B-Cell gene expression comparison between Wiskott-Aldrich syndrome protein KO mice and WT mice

ABSTRACT: This experiment intended to define differential gene expression between germinal center B cells expressing or not the Wiskott-Aldrich syndrome protein in mice. Sequencing was obtained on an Illumina HiSeq2500 system from Dark Zone GCB (DAPI-CD19+ GL7+IgD-CXCR4highCD86low) purified from CTL and GCBcWKO mice (n=4).

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Rémi Fritzen

PROVIDER: E-MTAB-10056 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Critical role of WASp in germinal center tolerance through regulation of B cell apoptosis and diversification.

Descatoire Marc M Fritzen Remi R Rotman Samuel S Kuntzelman Genevieve G Leber Xavier Charles XC Droz-Georget Stephanie S Thrasher Adrian J AJ Traggiai Elisabetta E Candotti Fabio F

Cell reports 20220301 10

A main feature of Wiskott-Aldrich syndrome (WAS) is increased susceptibility to autoimmunity. A key contribution of B cells to development of these complications has been demonstrated through studies of samples from affected individuals and mouse models of the disease, but the role of the WAS protein (WASp) in controlling peripheral tolerance has not been specifically explored. Here we show that B cell responses remain T cell dependent in constitutive WASp-deficient mice, whereas selective WASp ...[more]

PMID: 35263577

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