Unknown,Transcriptomics,Genomics,Proteomics

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Affect of antiviral ribavirin on YFV WT and vaccine strains.


ABSTRACT: Live-attenuated viral vaccines have been successfully used to combat infectious disease for decades but due to their empirical derivation, little is known about their mechanisms of attenuation. This lack of understanding makes the development of next generation live attenuated vaccines difficult. The success of the 17D vaccine and availability of the parent virus, Asibi, makes it an excellent model to understand the molecular basis of attenuation of a live attenuated vaccine and the effects of viral diversity on vaccine function. Due to the differences in genetic diversity between WT Asibi virus and its 17D vaccine derivative, we investigated the changes in genetic diversity of 17D and Asibi viruses following treatment with ribavirin.

INSTRUMENT(S): Illumina HiSeq 1500

ORGANISM(S): Yellow fever virus

SUBMITTER: Alan Barrett 

PROVIDER: E-MTAB-8504 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Attenuation of Live-Attenuated Yellow Fever 17D Vaccine Virus Is Localized to a High-Fidelity Replication Complex.

Davis Emily H EH   Beck Andrew S AS   Strother Ashley E AE   Thompson Jill K JK   Widen Steven G SG   Higgs Stephen S   Wood Thomas G TG   Barrett Alan D T ADT  

mBio 20191022 5


The molecular basis of attenuation for live-attenuated vaccines is poorly understood. The yellow fever (YF) 17D vaccine virus was derived from the wild-type, parental strain Asibi virus by serial passage in chicken tissue and has proven to be a very safe and efficacious vaccine. We have previously shown that wild-type Asibi is a typical RNA virus with high genetic diversity, while the 17D vaccine virus has very little genetic diversity. To investigate this further, we treated Asibi and 17D virus  ...[more]

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