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Structural and non-structural genes contribute to the genetic diversity of RNA viruses

ABSTRACT: With the advent of advanced sequencing technology, studies of RNA viruses have shown that genetic diversity contribute to both attenuation and virulence. The differences in genetic diversity of wild-type Asibi virus and 17D-204 vaccine provides an unique opportunity to investigate RNA population theory in the context of a well described live attenuated vaccine. Utilizing infectious clone-derived viruses containing some of the amino acid substitutions that differentiate yellow fever wild-type Asibi strain from 17D vaccine and recovered in a controlled experiment, establishes that the genetic diversity differences that exist between wild-type Asibi and 17D-204 vaccine viruses are not influenced by either different passage history or source of samples, but rather resulted from the attenuation of wild-type Asibi virus to yield the 17D vaccine sub-strains.

INSTRUMENT(S): Illumina HiSeq 1500

ORGANISM(S): Yellow fever virus

SUBMITTER: Steve Widen

PROVIDER: E-MTAB-6614 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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