Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

38 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Maximizing linear amplification of nucleic acids with optimized T7 promoters

ABSTRACT: Linear amplification of RNA by T7 bacteriophage polymerase is widely used in molecular biology. We performed 5’RACE-Seq to identify T7 promoter variants with enhanced transcriptional activity that generate up to five-fold higher RNA output in large scale synthesis reactions. In single-cell RNA-Sequencing, optimized T7 promoters facilitate library preparation, and substantially increase library complexity and the number of expressed genes detected per cell, highlighting a particular value for bioanalytical applications

INSTRUMENT(S): Illumina MiniSeq

ORGANISM(S): Homo sapiens

SUBMITTER: Ramon Oliveira Vidal

PROVIDER: E-MTAB-8508 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Maximizing linear amplification of nucleic acids with optimized T7 promoters

Project description:Maximizing linear amplification of nucleic acids with optimized T7 promoters

| PRJEB35325 | ENA

Combined Solution and Crystal Methods Reveal the Electrostatic Tethers That Provide a Flexible Platform for Replication Activities in the Bacteriophage T7 Replisome

Project description:We use Illumina sequencing to monitor mutations in the bacteriophage T7 genome in the presence of T7 DNA polymerase that has an altered exonuclease active site. These alterations include mutation of key residues in the exonuclease active site.

2020-01-01 | GSE138098 | GEO

Escherichia phage T7

Project description:Bacteriophage T7 Genome Sequencing of Mutated Genomes

| PRJNA187454 | ENA

Effect of ligase on T7 based RNA linear amplification

Project description:Zhao et al. Amplification Table 3 This experiment was designed to evaluate the effect of ligase used in the second strand cDNA synthesis on the fidelity of T7 based RNA linear amplification. BC2 total RNA was amplified with or without ligase using Affymetrix protocol. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

2005-11-04 | GSE3561 | GEO

Effect of column cleanup on T7 based RNA linear amplification

Project description:Zhao et al. Amplification Table 4 This experiment was designed to evaluate the effect of column cleanup on the fidelity and yield of T7 based RNA linear amplification. BC2 total RNA was amplified using the Jeffrey lab protocol with or without G50 cleanup. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

2005-11-04 | GSE3562 | GEO

1 Enhanced assembly of bacteriophage T7 produced in cell-free reactions under simulated microgravity

Project description:On-demand biomanufacturing has the potential to improve healthcare and self- sufficiency during space missions. Cell-free transcription and translation reactions combined with DNA blueprints can produce promising therapeutics like bacteriophages and virus-like particles. However, how space conditions affect the synthesis and self-assembly of such complex multi- protein structures is unknown. Here, we characterize the cell-free production of infectious bacteriophage T7 virions under simulated microgravity. Rotation in a 2D-clinostat increased the number of infectious particles compared to static controls. Quantitative analyses by mass spectrometry, immuno-dot-blot and real-time PCR showed no significant differences in protein and DNA contents, suggesting enhanced self-assembly of T7 phages in simulated microgravity. While the effects of genuine space conditions on the cell-free synthesis and assembly of bacteriophages remain to be investigated, our findings support the vision of a cell-free synthesis-enabled “astropharmacy”.

2024-03-13 | PXD044176 | Pride

Rhodococcus sp. T7 strain:T7

Project description:Rhodococcus sp. T7 strain:T7 Genome sequencing and assembly

| PRJNA598817 | ENA

Escherichia phage T7 strain:T7

Project description:Escherichia phage T7 strain:T7 Genome sequencing and assembly

| PRJNA733815 | ENA

Paenibacillus massiliensis T7 strain:T7

Project description:Paenibacillus massiliensis T7 strain:T7 Genome sequencing and assembly

| PRJNA202952 | ENA

Effect of ligase on T7 based RNA linear amplification

2005-11-03 | E-GEOD-3561 | biostudies-arrayexpress