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Genome-wide CRISPR knockout screen in Huh7.5.1 human hepatocytes for the identification of host factors of hepatitis A virus

ABSTRACT: A knockout cell library in Huh7.5.1 cells was generated by introducing a genome-scale CRISPR library (GeCKOv2, Addgene #1000000049) and subjected to hepatitis A virus infection (HM175/18f) to isolate virus-resistant mutant cells. Genomic DNA was isolated from the original and virus-selected mutant cell populations and abundance of guideRNA encoding sequences were measured by sequencing on an Illumina NextSeq (High Output).

INSTRUMENT(S): NextSeq 550

ORGANISM(S): Homo sapiens

SUBMITTER: Andreas Puschnik

PROVIDER: E-MTAB-8646 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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