Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Bulk RNAseq on hemocytes from stage 16 embryos and wandering 3rd instar larvae of Drosophila melanogaster

ABSTRACT: The aim of this experiment is to compare the transcriptome of Drosophila hemocytes at two developmental stages: in stage 16 embryo, mostly free from any immune challenge and in larvae, which grow in bacteria rich environment. Transgenes inducing the expression of RFP in the hemocytes were used to sort the hemocytes from the two stages before carrying out the sequencing.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Pierre Cattenoz

PROVIDER: E-MTAB-8702 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Temporal specificity and heterogeneity of Drosophila immune cells.

Cattenoz Pierre B PB Sakr Rosy R Pavlidaki Alexia A Delaporte Claude C Riba Andrea A Molina Nacho N Hariharan Nivedita N Mukherjee Tina T Giangrande Angela A

The EMBO journal 20200312 12

Immune cells provide defense against non-self and have recently been shown to also play key roles in diverse processes such as development, metabolism, and tumor progression. The heterogeneity of Drosophila immune cells (hemocytes) remains an open question. Using bulk RNA sequencing, we find that the hemocytes display distinct features in the embryo, a closed and rapidly developing system, compared to the larva, which is exposed to environmental and metabolic challenges. Through single-cell RNA ...[more]

PMID: 32162708

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Dataset Information

Bulk RNAseq on hemocytes from stage 16 embryos and wandering 3rd instar larvae of Drosophila melanogaster

Publications

Temporal specificity and heterogeneity of Drosophila immune cells.

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