Gene expression by E. coli during the production of styrene and when exposed to externally added styrene
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ABSTRACT: Transcriptomic studies of high-cell density fermentations of E. coli producing chemicals of relevance for biotechnology are limited. The aim of this study was to perform fermentations of an E. coli strain that had been engineered to produce styrene and examine the transcriptomic response. This was compared to an identical strain of E. coli that had the styrene pathway inactivated by the introduction of amino acid mutations in PAL2 and fdc1, the two enzymes required for styrene production. A further comparison was done by exposing E. coli cells to styrene added externally to the fermentation broth.
INSTRUMENT(S): Agilent high resolution microarray scanner, 3 L BioFlo®/CelliGen® 115 Bioreactor (New Brunswick Scientific)
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655
SUBMITTER: Joseph Webb
PROVIDER: E-MTAB-8703 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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