Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling by array of E. coli HMS174(DE3) to analyze the cellular response to high level cytoplasmic protein expression

ABSTRACT: This transcription profiling time course experiment was conducted in order to analyze the cellular response of E. coli HMS174(DE3)-pET30a-GFPmut3.1-Strep to high level cytoplasmic protein expression. Three biological replicates were generated by using a carbon limited exponential fed-batch cultivation.

ORGANISM(S): Escherichia coli

SUBMITTER: Peter Krempl

PROVIDER: E-MARS-23 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of E. coli HMS174(DE3) to analyze the cellular response to high level export of recombinant protein to the periplasm

Project description:This transcription profiling time course experiment was conducted in order to analyze the cellular response of E. coli HMS174(DE3)-pET30a-sSpAD-GFPmut3.1-Strep to recombinant protein export to the periplasm. Three biological replicates were generated by using a carbon limited exponential fed-batch cultivation.

2013-10-10 | E-MARS-22 | biostudies-arrayexpress

Transcription profiling by array of E. coli BL21(DE3)::TN7T7 -SOD to analyze the cellular response of this plasmid-free expression system to high level expression of recombinant human super-oxide-dismutase

Project description:This transcription profiling time course experiment was conducted in order to analyze the cellular response of the plasmid-free expression system E.coli BL21(DE3)::TN7<T7 -SOD> to high level expression of recombinant human super-oxide-dismutase (SOD).Three biological replicates were generated by using a carbon limited exponential fed-batch cultivation similar to industrial setups for large scale production. For induction of the system a single pulse of isopropyl-beta-D-galactoside (IPTG) yielding in a fully induced system is applied one doubling past feed start.

2013-05-31 | E-MARS-24 | biostudies-arrayexpress

Transcription profiling of E. coli BL21(DE3) to analyze the cellular response to high level expression of recombinant human super-oxide-dismutase (SOD)

Project description:This transcription profiling time course experiment was conducted in order to analyze the cellular response of E. coli BL21(DE3) to high level expression of recombinant human super-oxide-dismutase (SOD) on the transcriptional level. Three biological replicates were generated by using a carbon limited exponential fed-batch cultivation similar to industrial setups for large scale production. For induction of the system a single pulse of isopropyl-beta-D-galactoside (IPTG) yielding in a fully induced system is applied one doubling past feed start. In order to achieve a proper timeresolution of cellular responses sampling starts with a high frequency for the first hours past induction and decreases in the course of the experiment.

2011-09-30 | E-MARS-19 | biostudies-arrayexpress

Transcription profiling of E. coli HMS174(DE3)(pET30aNproGFPmut3.1) to analyze cellular response to limited induction with IPTG

Project description:This transcription profiling time course experiment was conducted in order to analyze the cellular response of the T7 RNA polymerase based E. coli expression system HMS174(DE3)(pET30aNproGFP) to a limited induction with isopropyl-beta-D-galactoside (IPTG). The used cell material was produced under well controlled and defined conditions in an exponential carbon limited fed-batch cultivation at a growth rate of 0.1 per hour. One doubling past feed start induction was started by continuously feeding limiting amounts of inducer gaining in a constant IPTG to cell dry weight ratio of 1µmol/g. Due to the low induction level a physiologically tolerable recombinant gene expression rate was generated and production period was significantly elongated. Non induced cells sampled at the time point of induction were used as reference and compared to cells subsequently sampled during the production period (sampling frequency 2 hours).

2010-03-26 | E-MARS-17 | biostudies-arrayexpress

Transcription profiling by array for comparison of industrial Escherichia coli strains on growth, transcriptome and proteome level in batch cultivations to identify strain specific features (subexperiment K-12 platform)

Project description:The objective of the current study was to evaluate the performance of frequently used E. coli K-12 strains HMS174 and RV308 in the context of industrial cultivations. To meet this objective, we carried out batch cultivations with high-glucose media in computer-controlled bioreactors and applied transcriptome analysis to interpret phenotypic attributes at the molecular level. The resulting enhancement of knowledge about host physiology is expected to contribute to the acceleration of improvements and optimizations in related biotech processes.

2013-08-19 | E-MARS-20 | biostudies-arrayexpress

Project description:The objective of the current study was to evaluate the performance of frequently used E. coli BL21 in the context of industrial cultivations. To meet this objective, we carried out batch cultivations with high-glucose media in computer-controlled bioreactors and applied transcriptome analysis to interpret phenotypic attributes at the molecular level. The resulting enhancement of knowledge about host physiology is expected to contribute to the acceleration of improvements and optimizations in related biotech processes.

2013-08-19 | E-MARS-21 | biostudies-arrayexpress

Fra1 silencing in MDA-MB-231 cells

Project description:Fra1 silencing in MDA-MB-231 cells

2013-07-24 | E-MTAB-1230 | biostudies-arrayexpress

Transcription profiling of Corynebacterium glutamicum grown in fermenter at different pH values.

Project description:Analysis of the transcriptomes of cell grown at different pH levels pH6.0, pH7.5, pH9.0; Cells were grown in a fermenter at different pH values to an oD600 between 4.5 to 11.5

2009-10-22 | E-MTAB-151 | biostudies-arrayexpress

Transcription profiling of Cadmium induced defects in the embryo 5 h after maternal Cd exposure in the absence or presence of Zn pretreatment

Project description:Cadmium (Cd) administered to female C57BL/6 mice on gestation day 8 induces a high incidence of anterior neural tube defects (exencephaly). This adverse effect can be attenuated by maternal pretreatment with zinc (Zn). In this study we used replicated microarray analysis to investigate gene expression changes induced in the embryo 5 h after maternal Cd exposure in the absence or presence of Zn pretreatment.

2006-04-11 | E-MEXP-529 | biostudies-arrayexpress

Microarray analysis of the effect of hsa-miR-127-3p on the gene expression of Hela cells under the stimulation with IFN-α

Project description:In order to explore the effect of hsa-miR-127-3p on the gene expression downstream of type I interferon signaling pathway, we used IFN-α to treat Hela cells (1000U/ml for 8 h) which were previously transfected with hsa-miR-127-3p mimics or various controls (mock transfection, negative control mimics or hsa-miR-127-3p mutant mimics). RNAs from the Hela cells were subjected to microarray analysis.

2021-10-08 | E-MTAB-10804 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data