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RNA-seq of small intestinal organoids treated with an inhibitor for the epigenetic modifier LSD1

ABSTRACT: To assess the role of LSD1 in mice small intestinal epithelium, small intestinal organoids were treated with an inhibitor for LSD1 (GSK-LSD1) and compared to untreated organoids. Similar to intestinal epithelium from mice with an intestinal epithelium specific LSD1-KO, paneth cells dissappear upon GSK-LSD1 treatment. We used these sequencing data to show that these small intestinal organoids have a similar phenotype as mice epithelium without LSD1.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Håvard Lindholm

PROVIDER: E-MTAB-9077 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium.

Zwiggelaar Rosalie T RT Lindholm Håvard T HT Fosslie Madeleine M Terndrup Pedersen Marianne M Ohta Yuki Y Díez-Sánchez Alberto A Martín-Alonso Mara M Ostrop Jenny J Matano Mami M Parmar Naveen N Kvaløy Emilie E Spanjers Roos R RR Nazmi Kamran K Rye Morten M Drabløs Finn F Arrowsmith Cheryl C Arne Dahl John J Jensen Kim B KB Sato Toshiro T Oudhoff Menno J MJ

Science advances 20200911 37

Intestinal epithelial homeostasis is maintained by adult intestinal stem cells, which, alongside Paneth cells, appear after birth in the neonatal period. We aimed to identify regulators of neonatal intestinal epithelial development by testing a small library of epigenetic modifier inhibitors in Paneth cell-skewed organoid cultures. We found that lysine-specific demethylase 1A (<i>Kdm1a/Lsd1</i>) is absolutely required for Paneth cell differentiation. <i>Lsd1</i>-deficient crypts, devoid of Panet ...[more]

PMID: 32917713

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