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RNA-seq of murine small intestinal organoids treated with type I PRMT inhibitor MS023 against untreated controls

ABSTRACT: Murine small intestinal organoids were cultured in the presence or absence of 5µM inhibitor MS023 (PubChem CID 92136227), which targets type I protein arginine methyltransferases Prmt1, Prmt3, Prmt4, Prmt6, Prmt8. Organoids were grown in culture media containing EGF, Noggin and R-spondin (ENR), media was changed after 48h, and organoids were harvested after 120h.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Håvard Lindholm

PROVIDER: E-MTAB-9291 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation.

Ostrop Jenny J Zwiggelaar Rosalie T RT Terndrup Pedersen Marianne M Gerbe François F Bösl Korbinian K Lindholm Håvard T HT Díez-Sánchez Alberto A Parmar Naveen N Radetzki Silke S von Kries Jens Peter JP Jay Philippe P Jensen Kim B KB Arrowsmith Cheryl C Oudhoff Menno J MJ

Frontiers in cell and developmental biology 20210121

Intestinal organoids are an excellent model to study epithelial biology. Yet, the selection of analytical tools to accurately quantify heterogeneous organoid cultures remains limited. Here, we developed a semi-automated organoid screening method, which we applied to a library of highly specific chemical probes to identify epigenetic regulators of intestinal epithelial biology. The role of epigenetic modifiers in adult stem cell systems, such as the intestinal epithelium, is still undefined. Base ...[more]

PMID: 33575256

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