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VEGFR2 Y949F (lung RNAseq - mice)

ABSTRACT: RNA sequencing of lung tissue from transgenic mice in order to investigate the effect of a single tyrosine to phenylalanine exchange in the endothelial receptor VEGFR2 at position Y949. This exchange creates a mouse with unleaky blood vessels which is an advantage in several diseases such as cancer and cardiovascular disease.

INSTRUMENT(S): Ion Torrent Proton

ORGANISM(S): Mus musculus

SUBMITTER: Miguel Sainz-Jaspeado

PROVIDER: E-MTAB-9163 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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VEGFR2 pY949 signalling regulates adherens junction integrity and metastatic spread.

Li Xiujuan X Padhan Narendra N Sjöström Elisabet O EO Roche Francis P FP Testini Chiara C Honkura Naoki N Sáinz-Jaspeado Miguel M Gordon Emma E Bentley Katie K Philippides Andrew A Tolmachev Vladimir V Dejana Elisabetta E Stan Radu V RV Vestweber Dietmar D Ballmer-Hofer Kurt K Betsholtz Christer C Pietras Kristian K Jansson Leif L Claesson-Welsh Lena L

Nature communications 20160323

The specific role of VEGFA-induced permeability and vascular leakage in physiology and pathology has remained unclear. Here we show that VEGFA-induced vascular leakage depends on signalling initiated via the VEGFR2 phosphosite Y949, regulating dynamic c-Src and VE-cadherin phosphorylation. Abolished Y949 signalling in the mouse mutant Vegfr2(Y949F/Y949F) leads to VEGFA-resistant endothelial adherens junctions and a block in molecular extravasation. Vessels in Vegfr2(Y949F/Y949F) mice remain sens ...[more]

PMID: 27005951

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