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RNA-seq of RBC depleted whole blood from COVID-19 patients and controls

ABSTRACT: Red blood cells (RBC) depleted whole blood from COVID-19 patients and controls was harvested and processed in order to performed 10X single cell RNA-seq. For COVID-19 patients 2 samples 10 days a part were analyzed.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Aymeric Silvin

PROVIDER: E-MTAB-9221 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Elevated Calprotectin and Abnormal Myeloid Cell Subsets Discriminate Severe from Mild COVID-19.

Silvin Aymeric A Chapuis Nicolas N Dunsmore Garett G Goubet Anne-Gaëlle AG Dubuisson Agathe A Derosa Lisa L Almire Carole C Hénon Clémence C Kosmider Olivier O Droin Nathalie N Rameau Philippe P Catelain Cyril C Alfaro Alexia A Dussiau Charles C Friedrich Chloé C Sourdeau Elise E Marin Nathalie N Szwebel Tali-Anne TA Cantin Delphine D Mouthon Luc L Borderie Didier D Deloger Marc M Bredel Delphine D Mouraud Severine S Drubay Damien D Andrieu Muriel M Lhonneur Anne-Sophie AS Saada Véronique V Stoclin Annabelle A Willekens Christophe C Pommeret Fanny F Griscelli Frank F Ng Lai Guan LG Zhang Zheng Z Bost Pierre P Amit Ido I Barlesi Fabrice F Marabelle Aurélien A Pène Frédéric F Gachot Bertrand B André Fabrice F Zitvogel Laurence L Ginhoux Florent F Fontenay Michaela M Solary Eric E

Cell 20200805 6

Blood myeloid cells are known to be dysregulated in coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2. It is unknown whether the innate myeloid response differs with disease severity and whether markers of innate immunity discriminate high-risk patients. Thus, we performed high-dimensional flow cytometry and single-cell RNA sequencing of COVID-19 patient peripheral blood cells and detected disappearance of non-classical CD14<sup>Low</sup>CD16<sup>High</sup> monocytes, accumulation of HLA ...[more]

PMID: 32810439

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