Primed and naïve marmoset pluripotent stem cells as a model for primate development
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ABSTRACT: We derived primed pluripotent stem cell cultures (PSCs) from marmoset blastocyst using conventional human PSC culture conditions (KSR/bFGF on feeders). Naïve marmoset PSCs were established by chemical resetting. In addition, we generated forebrain-derived cells from the frontal lobe of a marmoset neonate. Individual cells were manually isolated with a mouth pipette and subjected to single-cell full-lengths transcriptome profiling using a modified version of Smart-Seq2.
INSTRUMENT(S): Illumina HiSeq 4000
ORGANISM(S): Callithrix jacchus
SUBMITTER: Christopher Penfold
PROVIDER: E-MTAB-9349 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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