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Identification of the mRNAs interacting with the Nup59 nucleoporin using RNA-IP and microarrays in budding yeast

ABSTRACT: To specifically explore such mRNA localization events, we immunopurified the entire Nuclear Pore Complex scaffold using Nup59 as a bait and systematically analyzed all co-isolating mRNAs using Agilent microarrays in the budding yeast Saccharomyces cerevisiae. A mock IP was performed as a control experiment, using a yeast strain in which Nup59 was untagged. Two replicates of the Nup59 IP and of the mock IP were performed. The mRNAs with a significant enrichment (p<0.05 in the bayesian test of the LIMMA package) in the mock IP were removed from the further analyses of the Nup59-IP.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Frederic Devaux 

PROVIDER: E-MTAB-9521 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Co-translational assembly and localized translation of nucleoporins in nuclear pore complex biogenesis.

Lautier Ophélie O   Penzo Arianna A   Rouvière Jérôme O JO   Chevreux Guillaume G   Collet Louis L   Loïodice Isabelle I   Taddei Angela A   Devaux Frédéric F   Collart Martine A MA   Palancade Benoit B  

Molecular cell 20210409 11

mRNA translation is coupled to multiprotein complex assembly in the cytoplasm or to protein delivery into intracellular compartments. Here, by combining systematic RNA immunoprecipitation and single-molecule RNA imaging in yeast, we have provided a complete depiction of the co-translational events involved in the biogenesis of a large multiprotein assembly, the nuclear pore complex (NPC). We report that binary interactions between NPC subunits can be established during translation, in the cytopl  ...[more]

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