Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of mitochondrial mass fractions within SCA1+/PDGFRA+/CD31- fibroblasts from adult mouse hearts


ABSTRACT: In this project we explore the mitochondrial properties of SCA1+/PDGFRA+/CD31- (S+P+) cardiac fibroblasts (CF) from uninjured adult mouse hearts. We fractionated S+P+ cells on the basis of mitochondrial mass using MitoTracker green-FM and flow cytometry into three fractions: high, mid and low mitochondrial mass as well as total S+P+ cells. Bulk RNA-seq was then performed on each of the fractions.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Ralph Patrick 

PROVIDER: E-MTAB-9675 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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