RNA-seq of mitochondrial mass fractions within SCA1+/PDGFRA+/CD31- fibroblasts from adult mouse hearts
Ontology highlight
ABSTRACT: In this project we explore the mitochondrial properties of SCA1+/PDGFRA+/CD31- (S+P+) cardiac fibroblasts (CF) from uninjured adult mouse hearts. We fractionated S+P+ cells on the basis of mitochondrial mass using MitoTracker green-FM and flow cytometry into three fractions: high, mid and low mitochondrial mass as well as total S+P+ cells. Bulk RNA-seq was then performed on each of the fractions.
INSTRUMENT(S): Illumina HiSeq 2500
ORGANISM(S): Mus musculus
SUBMITTER: Ralph Patrick
PROVIDER: E-MTAB-9675 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
ACCESS DATA