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Whole transcriptome analysis of Sox17.Epi and Endo cells in mouse embryos

ABSTRACT: This experiment has been done to understand the dynamics of gene expression in Sox17.Epi and Endo cells using multiplex RNA-Seq technology. Sox17.Epi (Sox17^GFP+EpCAM+) and Sox17.Endo (Sox17^GFP+EpCAM-) cells were isolated by FACS from E9.5 mouse embryos. The spatial comparison between Sox17.Epi versus Sox17.Endo provides insight into which genes are differentially of specifically expressed in two populations.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Mark Magnuson

PROVIDER: E-MTAB-970 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dual lineage-specific expression of Sox17 during mouse embryogenesis.

Choi Eunyoung E Kraus Marine R C MR Lemaire Laurence A LA Yoshimoto Momoko M Vemula Sasidhar S Potter Leah A LA Manduchi Elisabetta E Stoeckert Christian J CJ Grapin-Botton Anne A Magnuson Mark A MA

Stem cells (Dayton, Ohio) 20121001 10

Sox17 is essential for both endoderm development and fetal hematopoietic stem cell (HSC) maintenance. While endoderm-derived organs are well known to originate from Sox17-expressing cells, it is less certain whether fetal HSCs also originate from Sox17-expressing cells. By generating a Sox17(GFPCre) allele and using it to assess the fate of Sox17-expressing cells during embryogenesis, we confirmed that both endodermal and a part of definitive hematopoietic cells are derived from Sox17-positive c ...[more]

PMID: 22865702

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