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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Effects of the Sox17∆50 mutation on SOX17 function in mouse embryonic biliary development

ABSTRACT: To determine the role of specific cis-regulatory elements within the Sox17 endoderm-preferential TSS2 promoter, we generated Sox17∆50 mutant animals and surveyed how this mutation altered Sox17 expression. EPCAM+/CXCR4+ endodermal cells were isolated from E10.5 Sox17∆50/∆50 and Sox17+/+ embryos (n = 1 of each genotype). Posterior foregut and midgut regions of embryos were dissected by removal of embryonic head, heart, tail, limb buds, posterior and dorsal body trunk. Sox17+/+ and Sox17Δ50/Δ50 cell samples were labelled with barcodes from 3'CellPlex Kit (10X Genomics) and subsequently pooled together for single isolation, library preparation and sequencing.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Jean-Philippe Cartailler

PROVIDER: E-MTAB-14000 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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