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Single cell TCR-seq profiling of T cells isolated from LNs of MC38 tumor-bearing mice

ABSTRACT: We investigate the single-cell landscape of the inflammatory mouse tumor model MC38, a C57BL/6 tumor cell line derived from colon adenocarcinoma. MC38 (diluted in HBSS and matrigel) was inoculated in the right unilateral flank (in the border of positions B2 and B3) of C57BL/6 mice (ref Study 16-3384 AV). Draining lymph nodes were taken one day after group-out (average 150-250 mm3 tumor size at day 0), approximately 14-19 days. Draining lymph nodes were dissociated and flow sorted accordingly to obtain the cells for 10x Chromium 5' Gene Expression Profiling and TCR sequencing.

INSTRUMENT(S): Illumina HiSeq 4000, Compute Cluster

ORGANISM(S): Mus musculus

SUBMITTER: Soren Muller

PROVIDER: E-MTAB-9734 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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