Microarray profiling of post-injury and uninjured mouse lung neutrophils from a thermal injury model
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ABSTRACT: Lungs were collected as soon as possible after thermal injury 30-40 mins post injury for neutrophil isolation. Lungs were minced in HBSS with 1% BSA (Sigma) and strained through a 40um nylon filter (BD). Samples were sorted using BD fusion machine. A 100um nozzle was used at a flow rate of 2.RNA was extracted from these neutrophils and microarray was performed.
ORGANISM(S): Mus musculus
SUBMITTER: Christopher Morehouse
PROVIDER: E-MTAB-9770 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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