Bulk RNA-seq of cerebral organoids investigating the role of CHD8 in cortical development
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ABSTRACT: We used cerebral organoids generated from wildtype and CHD8 +/- human ES cells to study the effects of CHD8, one of the top ASD risk genes, on early cortical development. CHD8 +/- hESC were generated using the CRISPR/Cas9 system to create a deletion within the helicase domain. Cerebral organoids were generated according to the protocol from Lancaster et al 2013 with minor modifications.
INSTRUMENT(S): Illumina HiSeq 2500
ORGANISM(S): Homo sapiens
SUBMITTER: Gaia Novarino
PROVIDER: E-MTAB-9933 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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