Unknown,Transcriptomics,Genomics,Proteomics

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Bulk RNA-seq of cerebral organoids investigating the role of CHD8 in cortical development


ABSTRACT: We used cerebral organoids generated from wildtype and CHD8 +/- human ES cells to study the effects of CHD8, one of the top ASD risk genes, on early cortical development. CHD8 +/- hESC were generated using the CRISPR/Cas9 system to create a deletion within the helicase domain. Cerebral organoids were generated according to the protocol from Lancaster et al 2013 with minor modifications.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Gaia Novarino 

PROVIDER: E-MTAB-9933 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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