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Amplicon-seq of an enhancer region targeted by CRISPR to assess the mutations introduced by different sgRNA guides

ABSTRACT: Following a CRISPR enhancer scan covering the GATA2 super-enhancer region, the top sgRNAs were selected for further inspection. MUTZ3 cells were thus treated with the selected sgRNAs and the region of interested was subjected to amplicons sequencing (amplicon-seq). To that end, we used the Illumina PCR-based custom amplicon sequencing method using the TruSeq Custom Amplicon index kit (Illumina). The same experiment was conducted in K562 cells, which do not harbor an inv(3)/t(3;3), to investigate the role of MYB in this enhancer in other leukemia settings

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Homo sapiens

SUBMITTER: Ruud Delwel

PROVIDER: E-MTAB-9945 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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