Unknown,Transcriptomics,Genomics,Proteomics

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Effects of CEBPA isoform expression on NB4 cells


ABSTRACT: Here we investigated the effects of CEBPA transcription factor expression on myeloid NB4 cells. The sequence of rat CEBPA was C-terminally fused to a promiscuous biotin ligase tag (BirA*) and NB4 cell lines were engineered to express the fusion protein under the control of a doxycycline inducible promoter. Three different NB4 cell lines were investigated that expressed (i) BirA* tag alone (ii) full length CEBPA isoform (P42) fused to BirA* (iii) truncated CEBPA isoform (P30) fused to BirA*. Cells were seeded in media supplemented with or without doxycycline.

ORGANISM(S): Homo sapiens

SUBMITTER: Achim Leutz 

PROVIDER: E-MTAB-9947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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