Effects of CEBPA isoform expression on NB4 cells
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ABSTRACT: Here we investigated the effects of CEBPA transcription factor expression on myeloid NB4 cells. The sequence of rat CEBPA was C-terminally fused to a promiscuous biotin ligase tag (BirA*) and NB4 cell lines were engineered to express the fusion protein under the control of a doxycycline inducible promoter. Three different NB4 cell lines were investigated that expressed (i) BirA* tag alone (ii) full length CEBPA isoform (P42) fused to BirA* (iii) truncated CEBPA isoform (P30) fused to BirA*. Cells were seeded in media supplemented with or without doxycycline.
ORGANISM(S): Homo sapiens
SUBMITTER: Achim Leutz
PROVIDER: E-MTAB-9947 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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