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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomic analysis of Brugia malayi-elicited alternatively activated macrophages

ABSTRACT: RNA-Seq expression profiling of flow cytometry sorted macrophages obtained from BALB/c and IL4Ra-/- mice via thioglycollate-elicitation or Brugia malayi implantation. BALBc implanted (BNe), BALBc Thioglycollate (BTG), IL4Ra-/- implanted (KNe) and IL4Ra-/- thioglycollate (KTG) groups were prepared. Each group consists of 3 biological replicates. Each biological replicate was obtained by pooling equal masses of total RNA from 3 individual mice.

ORGANISM(S): Mus musculus

SUBMITTER: Graham Thomas

PROVIDER: E-MTAB-995 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNA expression in thioglycollate and alternatively activated

Project description:Macrophage activation must be tightly controlled to prevent overzealous responses that cause self-damage. MicroRNAs have been shown to promote classical macrophage activation by blocking concomitant anti-inflammatory signals and transcription factors, but can also place restraints on activation by preventing excessive TLR-signalling. In contrast, the microRNA profile associated with alternatively activated macrophages and their role in regulating wound-healing or anti-helminthic responses has not yet been described. Utilizing an in vivo model of alternative activation, in which adult Brugia malayi nematodes are surgically implanted in the peritoneal cavity of mice, we examined the profile of microRNA expression in these alternatively activated macrophages and compared this to alternatively activated IL-4 receptor knockout macrophages and thioglycollate elicited macrophages. Peritoneal macrophages from BALB/c wild type or IL-4 receptor knockout mice were elicited with thioglycollate or using nemtodes (peritoneal implant of Brugia malayi). The latter leads to a population of alternatively activated macrophages. Microarray analysis was used to examine the microRNA profile of WT alternatively activated macrophages (n = 4), IL-4 receptor knockout alternatively activated macrophages (n = 4), WT thioglycollate elicited macrophages (n = 3) and IL-4 receptor knockout thioglycollate elicited macrophages (n = 3).

2012-08-07 | E-GEOD-35047 | biostudies-arrayexpress