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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Vibrio cholerae strains VCXB21 and VCXB21 pDel with added 0.6 mM chitobiose at early log phase

ABSTRACT: Strains VCXB21 and VCXB21 pDel were added 0.6 mM chitobiose at early log phase. Samples isolated after 30 min. cDNA from VCXB21 was prepared from 2 ug RNA and labeled with Cy3. cDNA from VCXB21pDel was prepared from 2 ug RNA and labeled with Cy5.

ORGANISM(S): Vibrio cholerae

SUBMITTER: Karin Meibom

PROVIDER: E-SMDB-2239 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The Vibrio cholerae chitin utilization program.

Meibom Karin L KL Li Xibing B XB Nielsen Alex T AT Wu Cheng-Yen CY Roseman Saul S Schoolnik Gary K GK

Proceedings of the National Academy of Sciences of the United States of America 20040201 8

Chitin, an insoluble polymer of GlcNAc, is an abundant source of carbon, nitrogen, and energy for marine microorganisms. Microarray expression profiling and mutational studies of Vibrio cholerae growing on a natural chitin surface, or with the soluble chitin oligosaccharides (GlcNAc)(2-6), GlcNAc, or the glucosamine dimer (GlcN)2 identified three sets of differentially regulated genes. We show that (i) ChiS, a sensor histidine kinase, regulates expression of the (GlcNAc)(2-6) gene set, including ...[more]

PMID: 14983042

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