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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MATT mapping of transposon insertions sites of H. pylori strain G27 GPS-cat library

ABSTRACT: Genomic DNA from pools of H. pylori strain G27 Clones as indicated (pools of 300 (300p) or insertions in specific mapped genes) were amplifed using the MATT method to label DNA adjacent to the site of transposon insertion with the primer pairs indicated. The left side of the transposon was labeled in the Cy3 channel (Primer S) and the right side of the transposon was labeled in the Cy5 channel (Primer N). The results of these experiments are published in Salama et al. 2004. J Bact. 186(23):7926-7935.

ORGANISM(S): Helicobacter pylori

SUBMITTER: Nina Salama

PROVIDER: E-SMDB-3048 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global transposon mutagenesis and essential gene analysis of Helicobacter pylori.

Salama Nina R NR Shepherd Benjamin B Falkow Stanley S

Journal of bacteriology 20041201 23

We have constructed a genome-saturating mutant library of the human gastric pathogen Helicobacter pylori. Microarray tracking of transposon mutants (MATT) allowed us to map the position of 5,363 transposon mutants in our library. While we generally found insertions well distributed throughout the genome, 344 genes had no detectable transposon insertions, and this list is predicted to be highly enriched for essential genes. Comparison to the essential gene set of other bacteria revealed a surpris ...[more]

PMID: 15547264

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