Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of Entamoeba histolytica plus Caco2 cells


ABSTRACT: Entamoeba histolytica is a protozoan parasite which causes colitis and liver abscesses. Using a genomic DNA microarray consisting of 1.6 - 2.0 kb genomic inserts we have generated a transcriptional profile of 1,971 unique parasite transcripts. The arrays in this experiment set were used to examine changes in the transcriptional profile of Entamoeba histolytica when the parasite interacts with colonic epithelial cells (Caco-2). A time course was used such that RNA was isolated from ameba alone and ameba + Caco-2 cells at 3hrs, 6hrs and 9hrs corresponding to 10%, 50% and 90% cell monolayer destruction. At least two biological experiments and three replicates were used for each time point.

ORGANISM(S): Entamoeba histolytica

SUBMITTER: Ryan Macfarlane 

PROVIDER: E-SMDB-3208 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Transcriptional profiling of Entamoeba histolytica trophozoites.

MacFarlane Ryan C RC   Shah Preetam H PH   Singh Upinder U  

International journal for parasitology 20050401 5


We have developed an Entamoeba histolytica genomic DNA microarray and used it to develop a transcriptional profile of 1,971 E. histolytica (HM-1:IMSS) genes. The arrays accurately detected message abundance and 31-47% of amebic genes were expressed under standard tissue culture conditions (levels detectable by Northern blot analysis or RT-PCR respectively). Genes expressed at high levels ( approximately 2% of total) included actin (8.m00351), and ribosomal genes (20.m00312). Moderately expressed  ...[more]

Similar Datasets

2005-07-22 | GSE2979 | GEO
2005-07-22 | E-GEOD-2979 | biostudies-arrayexpress
2005-07-06 | E-SMDB-3205 | biostudies-arrayexpress
2005-07-06 | E-SMDB-3207 | biostudies-arrayexpress
2006-01-03 | E-SMDB-3589 | biostudies-arrayexpress
2005-07-22 | GSE2978 | GEO
2006-03-07 | E-GEOD-4400 | biostudies-arrayexpress
2005-07-22 | E-GEOD-2978 | biostudies-arrayexpress
2006-03-08 | GSE4400 | GEO
2011-08-10 | GSE31295 | GEO