Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Vibro cholerae from rabbit ileal loop fluid 12 h post infection to identify differentially expressed genes


ABSTRACT: Transcriptional analysis of wild-type V. cholerae isolated from the rabbit ileal loop was performed by quickly pelleting bacteria from the fluid accumulated in the ileal loops after 12 h of infection. The bacteria were then resuspended in Trizol reagent and frozen on dry ice. The wild-type bacteria from two independent experiments in two different rabbits were analyzed. RNA was isolated from the Trizol agent, treated with DNaseI (Ambion, Austin, Texas, United States), and cleaned by using the RNeasy kit (Qiagen, Valencia, California, United States). Labeling of cDNA and microarray hybridizations were performed as described [Yiliz et al. 2001, Mol. Micro. 53: 497-515]. Microarrays were scanned with a GenePix 400A instrument (Axon Instruments), using the GENEPIX 5.0 software.

ORGANISM(S): Vibrio cholerae

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-3832 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

RpoS controls the Vibrio cholerae mucosal escape response.

Nielsen Alex Toftgaard AT   Dolganov Nadia A NA   Otto Glen G   Miller Michael C MC   Wu Cheng Yen CY   Schoolnik Gary K GK  

PLoS pathogens 20061001 10


Vibrio cholerae causes a severe diarrhoeal disease by secreting a toxin during colonization of the epithelium in the small intestine. Whereas the initial steps of the infectious process have been intensively studied, the last phases have received little attention. Confocal microscopy of V. cholerae O1-infected rabbit ileal loops captured a distinctive stage in the infectious process: 12 h post-inoculation, bacteria detach from the epithelial surface and move into the fluid-filled lumen. Designat  ...[more]

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