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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of fission yeast meiosis and sporulation

ABSTRACT: pat1 meiosis is very synchronous (see E-SNGR-2), but the use of a temperature shift and the prolonged nitrogen starvation can lead to artifacts. As a control for the pat1 experiment,non-sporulating pat1+ diploids were treated exactly as in the protocol for pat1 cells, with the exception that no nitrogen was added at the time of the temperature shift. Under these conditions the cells remain blocked in G1. As a reference we used a pool consisting of equal amount of RNA from all time points of the experiments. This experiment is part of a series, E-SNGR-2 to E-SNGR-7.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Cordelia Langford

PROVIDER: E-SNGR-6 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The transcriptional program of meiosis and sporulation in fission yeast.

Mata Juan J Lyne Rachel R Burns Gavin G Bähler Jürg J

Nature genetics 20020805 1

Sexual reproduction requires meiosis to produce haploid gametes, which in turn can fuse to regenerate a diploid organism. We have studied the transcriptional program that drives this developmental process in Schizosaccharomyces pombe using DNA microarrays. Here we show that hundreds of genes are regulated in successive waves of transcription that correlate with major biological events of meiosis and sporulation. Each wave is associated with specific promoter motifs. Clusters of neighboring genes ...[more]

PMID: 12161753

Similar Datasets

Transcription profiling of fission yeast meiosis and sporulation

Project description:pat1 meiosis is very synchronous (see E-SNGR-2), but the use of a temperature shift and the prolonged nitrogen starvation can lead to artifacts. As a control for the E-SNGR-2 experiment, non-sporulating pat1+ diploids were treated exactly as in the protocol for E-SNGR-2 cells. Under these conditions the cells re-enter the mitotic cycle. As a reference we used a pool consisting of equal amount of RNA from all time points of the experiments. This experiment is part of a series, E-SNGR-2 to E-SNGR-7.

2002-08-05 | E-SNGR-5 | biostudies-arrayexpress

Transcription profiling of fission yeast meiosis and sporulation

Project description:Transcriptional profile of fission yeast meiosis and sporulation. Fission yeast cells undergo meiosis and sporulation under conditions of nutritional stress, most frequently nitrogen starvation. This is a complex developmental process, which results in the formation of four spores that are highly resistant to environmental stress. We have carried out time courses of diploid fission yeast cells undergoing meiosis and sporulation. To achieve the best possible synchrony we have used thermosensitive mutants in the meiotic inhibitor pat1. We first pre-synchronized the cells in G1 by removal of nitrogen at the permissive temperature for pat1, and then induced meiosis by inactivating pat1 with a temperature shift. pat1-induced meiosis is a standard technique, resulting in a highly synchronous meiosis that is very similar to the normal process. The use of pat1 mutants and a temperature-shift may cause some artifacts, so these results should be viewed together with temperature shift controls and especially with the results of the time course done with wild type diploids, available under accession numbers E-SNGR-3 to E-SNGR-7. In all cases we used as reference a pool consisting of equal amounts of RNA from all time points of the experiment. This experiment describes the first biological replicate for pat1 using array design A-SNGR-2. Note that there are three other biological replicates, which use array design A-SNGR-1 in experiment E-SNGR-2.

2002-08-05 | E-SNGR-7 | biostudies-arrayexpress

Transcription profiling of fission yeast meiosis and sporulation

Project description:Fission yeast cells undergo meiosis and sporulation under conditions of nutritional stress, most frequently nitrogen starvation. This is a complex developmental process, which results in the formation of four spores that are highly resistant to environmental stress. We have carried out time course experiments with diploid fission yeast cells undergoing meiosis and sporulation. In this experiment we have used wild type diploids, and induced meiosis by removal of nitrogen from the medium. As a reference we used a pool consisting of equal amount of RNA from all time points of the experiments. This experiment is part of a series, with accession numbers E-SNGR-2 to E-SNGR-7.

2002-08-05 | E-SNGR-3 | biostudies-arrayexpress

Transcription profiling of fission yeast meiosis and sporulation

Project description:Study of the meiotic transcriptional profile of transcription factor mutants. The transcription factors atf21 and atf31 are highly induced during the meiotic divisions and required for proper spore formation. To identify possible targets of these transcription factors we compared the transcriptional profile of atf21 and atf31 mutants with that of wild type cells undergoing meiosis. We used haploid h90 cells for this experiment. Removal of nitrogen from the medium induces mating to form diploids, which immediately undergo meiosis and sporulation. This type of meiosis is not very synchronous. This experiment is part of a series, E-SNGR-2 to E-SNGR-7.

2002-08-05 | E-SNGR-4 | biostudies-arrayexpress

Replication origin mapping of mitotic and meiotic cells in different conditions

Project description:Identification of sites of replication initiation by copy number analysis, comparing samples before and after entry into S phase. Experiments were performed in the following strains and conditions for mitotic cells: cdc25-22 (temperature-sensitive mutation arrests cells at G2/M for synchrony, otherwise wild type), nitrogen-rich conditions; wild type, haploid and diploid, nitrogen-depletion conditions. Experiments were performed in the following strains and conditions for meiotic cells: pat1-114 diploid (temperature-sensitive mutation induces a synchronous meiosis, otherwise wild type), nitrogen-depletion and nitrogen-rich conditions; pat1-114 diploid with altered Cdc45 levels, nitrogen-depletion conditions.

2014-02-20 | GSE53879 | GEO

Rad51 binding in meiosis after undergoing S phase with different replication programs.

Project description:ChIP-on-chip analysis of Rad51 binding in cells induced to undergo synchronous meiosis after growth in continuous nitrogen-rich conditions or after nitrogen depletion. Comparison of pat1-114 cells grown in -N and +N conditions as well as pat1-114 cells with altered Cdc45 levels in -N conditions. pat1-114 rec12M-NM-^T mutants in -N and +N conditions were also evaluated to confirm that Rad51 binding is dependent on the Rec12 transesterase.

2014-02-20 | E-GEOD-53880 | biostudies-arrayexpress

Replication origin mapping of mitotic and meiotic cells in different conditions

2014-02-20 | E-GEOD-53879 | biostudies-arrayexpress

Pombe_ribosome_profiling_pat1_meiotic_cells

Project description:We applied in parallel RNA-Seq and Ribosome-profiling to S. pombe pat1 diploids undergoing meiosis and sporulation in a synchronous manner

2014-06-03 | E-MTAB-2179 | biostudies-arrayexpress

Pombe_ribosome_profiling_pat1_meiotic_cells

Project description:We applied in parallel RNA-Seq and Ribosome-profiling to S. pombe pat1 diploids undergoing meiosis and sporulation in a synchronous manner

2014-06-03 | E-MTAB-2265 | biostudies-arrayexpress

An RNA-binding protein Spo5 promotes meiosis II through the regulation of cyclin Cdc13

Project description:To determine the range of genes whose expression is regulated by Spo5 under nitrogen starbation, comparison of gene expression profiles between a wild-type strain and a deletion mutant of spo5, which encodes the meiosis specific RNA-binding protein Spo5. Cells of the wild-type (JY882) and spo5M-bM-^HM-^F (MA482) fission yeast strains were grown to the mid-log phase in liquid MM and shifted to nitrogen-free MMM-bM-^@M-^SN at 25M-BM-:C. The cells were harvested 6 h after this shift, the tempereture was raised to 34M-BM-:C. RNA was prepared from each sample after the temperature shift and analyzed on a DNA microarray covering 5,073 genes.

2013-07-01 | E-GEOD-43161 | biostudies-arrayexpress

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