Transcription profiling of fission yeast meiosis and sporulation

ABSTRACT: Transcriptional profile of fission yeast meiosis and sporulation. Fission yeast cells undergo meiosis and sporulation under conditions of nutritional stress, most frequently nitrogen starvation. This is a complex developmental process, which results in the formation of four spores that are highly resistant to environmental stress. We have carried out time courses of diploid fission yeast cells undergoing meiosis and sporulation. To achieve the best possible synchrony we have used thermosensitive mutants in the meiotic inhibitor pat1. We first pre-synchronized the cells in G1 by removal of nitrogen at the permissive temperature for pat1, and then induced meiosis by inactivating pat1 with a temperature shift. pat1-induced meiosis is a standard technique, resulting in a highly synchronous me

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Jurg Bahler 

PROVIDER: E-SNGR-7 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress