Barcode sequencing of Saccharomyces cerevisiae deletion libraries, SYBARIS project

ABSTRACT: A systematic approach allowing the identification of the molecular way-of-action of novel potential drugs represents the golden-tool for drug-discovery. While high-throughput screening technologies of large libraries is now well established, the assessment of the drug targets and mechanism of action is still under development. Taking advantage of the yeast model Saccharomyces cerevisiae, we herein applied BarSeq, a Next Generation Sequencing-based method to the analysis of both haploinsufficiency and homozygous fitness effects of a novel antifungal drug ('089') compared to the well-known antifungal ketoconazole. '089' was a novel compound identified in during a screen for antifungal drugs, as it was showing fungicidal effects, and able to affect the yeast fitness at the mitochondrial leve

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Shyamasree Saha 

PROVIDER: E-SYBR-6 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress