Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of Arabidopsis wild type and topoIV mutant plants at the end of a dark period and after re-illumination

ABSTRACT: A topoisomeras IV mutant in a flu background was identified in a mutant screen for ectopic activation of a reporter for singlet oxygen release. Gene expression was profiled on AGRONOMCS1 microarrays for wild type and the topoIV mutant at the end of an 8h dark period and after 30min of re-illumination.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Lars Hennig

PROVIDER: E-TABM-1076 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Many of the genes coding for secreted protein effectors are arranged in gene clusters in the genome of the biotrophic plant pathogen Ustilago maydis. The largest of these gene clusters, cluster 19A, encodes 24 secreted effectors. Deletion of the entire cluster results in severe attenuation of virulence. The generation and analysis strains carrying sub-deletions identified 9 genes significantly contributing to tumor formation after seedling infection. As the individual contributions of these genes to tumor formation were small, we studied the response of maize plants to the whole cluster mutant as well as to several individual mutants by array analysis. This revealed distinct plant responses, demonstrating that the respective effectors have discrete plant targets. Many of the genes coding for secreted protein effectors are arranged in gene clusters in the genome of the biotrophic plant pathogen Ustilago maydis. The largest of these gene clusters, cluster 19A, encodes 24 secreted effectors. Deletion of the entire cluster results in severe attenuation of virulence. The generation and analysis strains carrying sub-deletions identified 9 genes significantly contributing to tumor formation after seedling infection. As the individual contributions of these genes to tumor formation were small, we studied the response of maize plants to the whole cluster mutant as well as to several individual mutants by array analysis. This revealed distinct plant responses, demonstrating that the respective effectors have discrete plant targets. We used the Affymetrix maize genome array to analyze the transcriptional responses of maize to cluster 19A mutants and individual sub-deletions for the cluster 19A genes tin1, tin3, tin4 and tin5. We found plant responses to the mutants were significantly different although the macroscopic phenotypes of the individual mutants were very similar. U. maydis infected parts of maize seedling leaves were dissected 4 days after inoculation with strain SG200∆19A, SG200∆tin1, SG200∆tin3, SG200∆tin4 and SG200∆tin5, respectively. We previously submitted data of maize leaves that were treated with the progenitor wild type strain SG200 as well as mock-infections under identical experimetal conditions (GEO: GSE10023). These data served as controls for this experiment.

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Transcription profiling by array of Arabidopsis wild type and topoIV mutant plants at the end of a dark period and after re-illumination

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