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Transcriptiion profiling by array of human ratiometric mixes of high quality RNA from different tissues using different labeling protocols to provide a mechanism for assessing process improvement

ABSTRACT: Ratiometric controls were prepared from commercial sources of high quality RNA from human tissues with distinctly different expression patterns. The samples were processed using six different target labeling protocols and replicate datasets were generated on high density gene expression microarrays. The area under the curve from receiver operating characteristic plots was calculated as a measure of diagnostic performance. The reliable region of the dynamic range was derived from log ratio deviation plots made for each dataset. These samples and metrics provide a mechanism for assessing process improvement that can be used by clinical laboratories conducting microarray assays.

Additional processed data files are available on the FTP site for this experiment in E-TABM-1091.additional.zip.

ORGANISM(S): Homo sapiens

SUBMITTER: P Scott Pine

PROVIDER: E-TABM-1091 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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