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Transcription profiling of rat mixed-tissue RNA samples using different labeling protocols

ABSTRACT: At one site (#10), three different batches of MTRRM (see E-TABM-16), were labeled with two different kits (Enzo and Affymetrix) and hybridized to two different Affymetrix Arrays (RAE230A and RAE230_2).

ORGANISM(S): Rattus norvegicus

SUBMITTER: P Scott Pine

PROVIDER: E-MEXP-1563 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Use of diagnostic accuracy as a metric for evaluating laboratory proficiency with microarray assays using mixed-tissue RNA reference samples.

Pine P S PS Boedigheimer M M Rosenzweig B A BA Turpaz Y Y He Y D YD Delenstarr G G Ganter B B Jarnagin K K Jones W D WD Reid L H LH Thompson K L KL

Pharmacogenomics 20081101 11

Effective use of microarray technology in clinical and regulatory settings is contingent on the adoption of standard methods for assessing performance. The MicroArray Quality Control project evaluated the repeatability and comparability of microarray data on the major commercial platforms and laid the groundwork for the application of microarray technology to regulatory assessments. However, methods for assessing performance that are commonly applied to diagnostic assays used in laboratory medic ...[more]

PMID: 19018728

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