Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human bronchial epithelial cells from cultures exposed to cigarette smoke or filtered air

ABSTRACT: Cell cultures from three human non-smoker donors were exposed 1:50 dose (1.84ug/cm2) of diluted mainstream cigarette smoke or filtered air for 1h. Cells from four replicate inserts for each dose/time-point were pooled and placed in TRIzol either immediately after exposure or following a 5h or 23h recovery period after the cultures were returned to an incubator at 37¬∫C, 5% CO2. The ¬ëno treatment¬í control (NTC) remained in the incubator and received neither smoke nor air. In order to perform robust statistical analyses three independent replicate experiments were performed on each of the three donors

ORGANISM(S): Homo sapiens

SUBMITTER: Heather Maunders

PROVIDER: E-TABM-127 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Human bronchial epithelial cell transcriptome: gene expression changes following acute exposure to whole cigarette smoke in vitro.

Maunders Heather H Patwardhan Sudhanshu S Phillips Jeremy J Clack Aaron A Richter Audrey A

American journal of physiology. Lung cellular and molecular physiology 20070112 5

Cigarette smoke is a complex mixture of more than 4,000 constituents. Its effects on cell biology are poorly understood, partly because whole smoke exposure in vitro is technically challenging. To investigate the effects of smoke on cell signaling and function, a three-dimensional air-liquid interface model of tracheobronchial epithelium, grown from primary human lung epithelial cells, was exposed to air or whole mainstream cigarette smoke for 1 h in a purpose-designed chamber. Gene expression p ...[more]

PMID: 17220372

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Project description:Background: In classrooms high concentrations of particulate matter PM10 were measured. It is unknown whether the hazard of indoor particles is similar to that of the better studied outdoor particles. This study therefore analyzed adverse biological effects of classroom in comparison to outdoor PM10. Methods: Samples were taken from six schools during teaching hours. Genome-wide gene expression in human bronchial BEAS-2B epithelial cells was analyzed, and regulated genes were verified by quantitative PCR. Polycyclic aromatic hydrocarbons (PAH), endotoxin, and cat allergen Fel d 1 were analyzed with standard methods. Enhancement of allergic reactivity by PM10 was confirmed with CD63 upregulation in human primary basophils. Acceleration of human blood coagulation was determined with supernatants of PM10-exposed human peripheral blood monocytes. Results: Indoor PM10 induced SERPINB2 (involved in blood coagulation) and inflammatory genes (like CXCL6, CXCL1, IL6, IL8, all p<0.001). Outdoor PM10 induced xenobiotic metabolizing enzymes (CYP1A1, CYP1B1, TIPARP, all p<0.001). The induction of inflammatory genes by indoor PM10 could be explained by endotoxin (indoor 128.5M-BM-142.2EU/mg versus outdoor 13.4M-BM-121.5EU/mg, p<0.001), the induction of CYP by outdoor PAH (indoor 8.3M-BM-14.9ng/mg versus outdoor 16.7M-BM-115.2ng/mg, p<0.01). The induction of SERPINB2 was confirmed by a more rapid human blood coagulation (p<0.05). Indoor PM10 had no effect on the allergic reactivity from human primary basophils, except in cat allergic individuals. This was explained by varying Fel d 1 concentrations in indoor PM10 (p<0.001). Conclusions: Indoor PM10, compared to outdoor PM10, was 6 times higher, had a different composition, and on an equal weight basis induced more inflammatory and allergenic reactions, and accelerated blood coagulation. Outdoor PM10 had significantly lower effects, but induced detoxifying enzymes. Therefore, preliminary interventions for the reduction of classroom PM10 seem reasonable, perhaps by intensified ventilation. For genome-wide gene expression analysis, BEAS-2B cells (passage 41) were incubated with 10M-BM-5g/ml PM10 (school 4 indoor and outdoor) for 4, 10 or 24h, all in triplicate. experiment type : time course

Dataset Information

Transcription profiling of human bronchial epithelial cells from cultures exposed to cigarette smoke or filtered air

Publications

Human bronchial epithelial cell transcriptome: gene expression changes following acute exposure to whole cigarette smoke in vitro.

Similar Datasets

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