Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Streptococcus pneumoniae after incubation with host respiratory tract epithelial cells

ABSTRACT: In order to study pneumococcal gene expression changes after incubation with host epithelial cells, logarithmic-phase grown S. pneumoniae strains (82 and R6) were incubated with host epithelial cells (A549 and ETEC) in T75 cell culture flasks at an MOI range of 10:1 ~ 50:1. After incubation for 1 h or 3 h, bacterial solution was removed and 7 ml of host cell lysis buffer which can remove host cells and stablize bacterial RNA was added immediately. Bacteria incubated with antibiotic-free media (MEM or DMEM) with 1% (v/v) FBS for the same time periods were used as medium controls. The RNA samples were isolated with RiboPure Bacteria kit (Ambion) or RNeasy Minikit (Qiagen), and purified with DNase I treatment. cDNA samples were synthesized and labeled with biotin-dUTP (samples) and fluorescein-dUTP (Enzo) (medium controls), respectively, by using a LabelStar Array kit (Qiagen). Divergent labeled samples were hybridized to the same microarray slide by using a Two-Color Nucleic Acid Microarray Toolkit (Invitrogen). The scattered light signals of Resonance Light Scattering (RLS) particles Ag and Au were captured with a GSD-501 RLS imager using an ICS-501 (version 2.3) Image Capture System (Invitrogen). The gridding of spots was performed with ArrayVision 8.3 (Imaging Research).

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER: Xin-Ming Song

PROVIDER: E-TABM-187 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Microarray analysis of Streptococcus pneumoniae gene expression changes to human lung epithelial cells.

Song Xin-Ming XM Connor Wayne W Jalal Shakiba S Hokamp Karsten K Potter Andrew A AA

Canadian journal of microbiology 20080301 3

Streptococcus pneumoniae infection starts from the respiratory tract where interaction with host epithelial cells occurs. To gain more insights on pneumococcal pathogenesis, an oligonucleotide (oligo)-based microarray was used to investigate gene expression changes of one serotype 3 encapsulated pathogenic S. pneumoniae strain 82 and one unencapsulated avirulent S. pneumoniae strain R6 upon exposure to human lung epithelial cells (A549) for 1 and 3 h, respectively. We observed that genes associa ...[more]

PMID: 18388990

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Dataset Information

Transcription profiling of Streptococcus pneumoniae after incubation with host respiratory tract epithelial cells

Publications

Microarray analysis of Streptococcus pneumoniae gene expression changes to human lung epithelial cells.

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