Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

47 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of bone marrow derived cells from mice stimulated with RANKL to generate osteoclasts in a time series

ABSTRACT: Bone marrow derived cells stimulated with RANKL (receptor activator of nuclear factor kappa B ligand, also known as Tumor necrosis factor ligand superfamily member 11) to generate osteoclasts.

ORGANISM(S): Mus musculus

SUBMITTER: Rajeev Aurora

PROVIDER: E-TABM-212 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Systems level analysis of osteoclastogenesis reveals intrinsic and extrinsic regulatory interactions.

Kiesel Jennifer J Miller Cheryl C Abu-Amer Yousef Y Aurora Rajeev R

Developmental dynamics : an official publication of the American Association of Anatomists 20070801 8

Osteoclasts are bone-resorbing cells derived from the myeloid lineage that play a central role in bone remodeling and inflammatory bone erosion diseases. The receptor activator of NF-kappaB ligand (RANKL) produced by osteoblasts and activated immune cells initiates the development of osteoclasts in the bone marrow. Using time series gene expression data, the intrinsic processes and the extrinsic factors that control osteoclastogenesis were identified. The gene expression profiles display distinc ...[more]

PMID: 17584858

Similar Datasets

Primary myeloid cell proteomics and transcriptomics: importance of β-tubulin isotypes for osteoclast function

Project description:Among hematopoietic cells, osteoclasts (Oc) and immature dendritic cells (Dc) are closely related myeloid cells with distinct functions; Oc participate skeleton maintenance while Dc sample the environment for foreign antigens. Such specificities rely on profound modifications of gene and protein expression during Oc and Dc differentiation. We provide global proteomic and transcriptomic analyses of primary mouse Oc and Dc, based on original SILAC and RNAseq data. We established specific signatures for Oc and Dc including genes and proteins of unknown functions. In particular, we showed that Oc and Dc have the same alpha and beta tubulin isotypes repertoire but that Oc express much more beta tubulin isotype Tubb6 (also known as Tubulin beta class V). In both mouse and human Oc, we demonstrate that elevated expression of Tubb6 in Oc is necessary for correct podosomes organization and thus for the structure of the sealing zone, which sustains the bone resorption apparatus. Hence, lowering Tubb6 expression hindered Oc resorption activity. Overall, we highlight here potential new regulators of Oc and Dc biology and illustrate the functional importance of the tubulin isotype repertoire in the biology of differentiated cells.

2020-09-16 | PXD018832 | Pride

Modulation of murine bone marrow derived dendritic cells by IL-4

Project description:The archetypal Th2 cytokine interleukin 4 (IL-4) has previously been shown to alternatively activate dendritic cells (DCs) both in vitro and in vivo.To more fully understand the impact of IL-4 dependent alternative activation of DCs, we used mRNA microarrays to define their transcriptional profile. RNAs were extracted from bone marrow-derived dendritic cells (BMDCs). Two groups of samples were taken, with three biological replicates in each: control untreated, and treated with recombinant-IL4 (at 20 ng/ml).

2015-04-28 | E-GEOD-59868 | biostudies-arrayexpress

Transcription profiling by array of mouse promylocyte cell line induced to differentiate over four days

Project description:The Mouse Promylocyte (MPRO) cell line was induced to differentiate over four days during which time MYC expression decreases. MYC is required for rDNA transcription; by looking at global gene expression under these conditions, our aim was to correlate the expression of Pol I related/specific genes with that of MYC as well as contrast to those genes specific to Pol II and III transcription.

2011-06-01 | E-MEXP-2786 | biostudies-arrayexpress

Transcription profiling by array of human CD14+ monocytes treated with cytokines IL-34 and mCSF to determine the effects on momocyte differentiation

Project description:IL-34 and mCSF effects on human monocyte differentiation

2011-05-18 | E-TABM-999 | biostudies-arrayexpress

Microarray analysis of WT bone marrow myeloid progenitors, BM cultured with GM-CSF and M-CSF, and monocytes treated with GM-CSF

Project description:Analysis of genes induced in DC precursors and in BM cells and monocytes treated with GM-CSF For progenitor arrays, bone marrow progenitors (CMP, GMP, CDP, and pre-cDC) were harvested from WT C57Bl/6 mice. For culture arrays, BM was cultured in the presence of GM-CSF or M-CSF and adherent and non-adherent cells sorted. For monocyte cultures, sorted BM monocytes were treated with GM-CSF for 0, 24 or 48 hours.

2012-05-20 | E-GEOD-37029 | biostudies-arrayexpress

Genome-wide microRNA expression induced by TNF

Project description:We sequenced microRNAs from bone marrow derived macrophages derived from the control (WT) and RBP-J conditional knockout mice (RBP-J KO; Rbpjf/f; LysM Cre). Examination of differential microRNA expression levels induced by TNF as well as regulated by RBP-J in bone marrow derived macrophages.

2016-07-22 | E-GEOD-72966 | biostudies-arrayexpress

Genome-wide expression profiling in bone marrow-derived macrophage (BMM) cells upon RANKL treatment

Project description:To screen for altered gene expression during osteoclastogenesis, RANKL-treated BMM cells were subjected to gene expression profiling. BMM cells were treated with M-CSF and RANKL for 0, 1, 2 and 3 days. Total RNA was isolated and analyzed by gene expression microarray using the MouseRef-8 v2.0 Expression BeadChip.

2014-07-30 | E-GEOD-57468 | biostudies-arrayexpress

Expression data from wild-type control and Dnmt3a knockout osteoclast

Project description:Epigenetic regulation is a fundamental mechanism mediating various cellular processes. However, epigenetic mechanisms in osteoclastogenesis remain to be elucidated. We performed microarray analysis to investigate gene expression in osteoclasts derived from wild-type and Dnmt3aknockout mice. In vitro osteoclast culture were performed using wild-type control and Dnmt3a knockout bone marrow-derived monocyte/macrophage precursor cells.

2015-02-23 | E-GEOD-59387 | biostudies-arrayexpress

Transcription profiling by array of mouse promyelocyte granulocytes with activated MYC-ER protein

Project description:The mouse promyelocyte (MPRO) granulocyte cell model expressing the MYC-ER fusion protein encoding transgene was used as a system to perform gene expression profiling in a granulocytic cell setting of low endogenous MYC expression (differentiated MYC-ER MPROs, D4) where we then activated the MYC-ER protein for 24 h with 4-OHT (D5T) as compared to a EtOH vehicle treated control (D5E).

2011-06-01 | E-MEXP-2794 | biostudies-arrayexpress

Zbtb46 expression distinguishes classical dendritic cells and their committed progenitors from other immune lineages

Project description:This SuperSeries is composed of the following subset Series: GSE37028: Microarray analysis of Zbtb46 KO CD4+ Splenic DCs and bone marrow erythroid progenitors GSE37029: Microarray analysis of WT bone marrow myeloid progenitors, BM cultured with GM-CSF and M-CSF, and monocytes treated with GM-CSF Refer to individual Series

2012-05-20 | E-GEOD-37030 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data