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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of femoral bone tissue from red junglefowl and domestic White Leghorn chicken

ABSTRACT: The purpose of this experiment was to compare gene expression in femoral bone from red junglefowl and White Leghorn chicken (five of each sex and population). Total RNA was prepared from the femoral midshaft and 20 µg RNA was used in each cDNA synthesis reaction. In hybridization a common reference design was used and each sample was subjected to dye-swap hybridization, rendering a total number of 40 hybridizations for the 20 samples. These experiments were approved by the animal research ethics committee in Gothenburg, Sweden (approvals 256/99 and 147-2001).

ORGANISM(S): Gallus gallus

SUBMITTER: Carl-Johan Rubin

PROVIDER: E-TABM-241 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential gene expression in femoral bone from red junglefowl and domestic chicken, differing for bone phenotypic traits.

Rubin Carl-Johan CJ Lindberg Johan J Fitzsimmons Carolyn C Savolainen Peter P Jensen Per P Lundeberg Joakim J Andersson Leif L Kindmark Andreas A

BMC genomics 20070702

<h4>Background</h4>Osteoporosis is frequently observed among aging hens from egg-producing strains (layers) of domestic chicken. White Leghorn (WL) has been intensively selected for egg production and it manifests striking phenotypic differences for a number of traits including several bone phenotypes in comparison with the wild ancestor of chicken, the red junglefowl (RJ). Previously, we have identified four Quantitative Trait Loci (QTL) affecting bone mineral density and bone strength in an in ...[more]

PMID: 17605776

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