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Comparative genomic hybridization of 30 ovarian cancer cell lines reveals amplification of PVT1 contributes to the pathophysiology of ovarian and breast cancer

ABSTRACT: DNA from 30 cultured ovarian cancer cell lines was extracted with Promega kits and hybridize to arrays. Arrays comprised of 1860 BACs selected to include genes known to be involved in cancer pathogenesis and 400 BACs selected to tile across 13 Mbp at 3q26, 15 Mbp at 8q24, and 30 Mbp at 20q centered on regions of recurrent amplification associated with reduced survival duration.

ORGANISM(S): Homo sapiens

DISEASE(S): ovarian cancer

SUBMITTER: Yinghui Guan

PROVIDER: E-TABM-246 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Amplification of PVT1 contributes to the pathophysiology of ovarian and breast cancer.

Guan Yinghui Y Kuo Wen-Lin WL Stilwell Jackie L JL Takano Hirokuni H Lapuk Anna V AV Fridlyand Jane J Mao Jian-Hua JH Yu Mamie M Miller Melinda A MA Santos Jennifer L JL Kalloger Steve E SE Carlson Joseph W JW Ginzinger David G DG Celniker Susan E SE Mills Gordon B GB Huntsman David G DG Gray Joe W JW

Clinical cancer research : an official journal of the American Association for Cancer Research 20071001 19

<h4>Purpose</h4>This study was designed to elucidate the role of amplification at 8q24 in the pathophysiology of ovarian and breast cancer because increased copy number at this locus is one of the most frequent genomic abnormalities in these cancers.<h4>Experimental design</h4>To accomplish this, we assessed the association of amplification at 8q24 with outcome in ovarian cancers using fluorescence in situ hybridization to tissue microarrays and measured responses of ovarian and breast cancer ce ...[more]

PMID: 17908964

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