Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of SET-2 deletion and wild type yeast on sense and anti-sense arrays


ABSTRACT: The presence of Set2-mediated methylation of H3K36 (K36me) correlates with transcription frequency throughout the yeast genome. K36me targets the Rpd3S complex to deacetylate transcribed regions and suppress cryptic transcription initiation at certain genes. Here, using a genome-wide approach, we report that the Set2-Rpd3S pathway is generally required for controlling acetylation at coding regions. When using acetylation as a functional read-out for this pathway, we discovered that longer genes and, surprisingly, genes transcribed at lower frequency exhibit a stronger dependency. Moreover, a systematic screen using high resolution tiling microarrays allowed us to identify a group of genes that rely on Set2-Rpd3S to suppress spurious transcripts. Interestingly, most of these genes are within the group that depend the same pathway to maintain a hypo-acetylated state at coding regions. These data highlight the importance of using the functional readout of histone codes to define the roles of specific pathways.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Bing Li 

PROVIDER: E-TABM-265 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Infrequently transcribed long genes depend on the Set2/Rpd3S pathway for accurate transcription.

Li Bing B   Gogol Madelaine M   Carey Mike M   Pattenden Samantha G SG   Seidel Chris C   Workman Jerry L JL  

Genes & development 20070601 11


The presence of Set2-mediated methylation of H3K36 (K36me) correlates with transcription frequency throughout the yeast genome. K36me targets the Rpd3S complex to deacetylate transcribed regions and suppress cryptic transcription initiation at certain genes. Here, using a genome-wide approach, we report that the Set2-Rpd3S pathway is generally required for controlling acetylation at coding regions. When using acetylation as a functional readout for this pathway, we discovered that longer genes a  ...[more]

Similar Datasets

2012-07-24 | E-GEOD-37158 | biostudies-arrayexpress
2012-07-24 | E-GEOD-37042 | biostudies-arrayexpress
2012-07-24 | E-GEOD-37102 | biostudies-arrayexpress
2008-09-04 | E-MEXP-1782 | biostudies-arrayexpress
2013-10-05 | E-MEXP-3995 | biostudies-arrayexpress
2010-01-13 | E-TABM-617 | biostudies-arrayexpress
2008-06-01 | E-MEXP-1080 | biostudies-arrayexpress
2008-06-01 | E-MEXP-1104 | biostudies-arrayexpress
2007-05-16 | E-TABM-260 | biostudies-arrayexpress
2009-03-26 | E-TABM-309 | biostudies-arrayexpress