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Chromatin immunoprecipitation of mouse hematopoietic cell lines and tissue-types to identify histone modification and transcription factor binding sites

ABSTRACT: Histone modification and transcription factor ChIP-chip in hematopoietic cell lines and tissue-types

ORGANISM(S): Mus musculus

SUBMITTER: Josette-Renee Landry

PROVIDER: E-TABM-431 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression of the leukemia oncogene Lmo2 is controlled by an array of tissue-specific elements dispersed over 100 kb and bound by Tal1/Lmo2, Ets, and Gata factors.

Landry Josette-Renée JR Bonadies Nicolas N Kinston Sarah S Knezevic Kathy K Wilson Nicola K NK Oram S Helen SH Janes Mary M Piltz Sandie S Hammett Michelle M Carter Jacinta J Hamilton Tina T Donaldson Ian J IJ Lacaud Georges G Frampton Jonathan J Follows George G Kouskoff Valerie V Göttgens Berthold B

Blood 20090126 23

The Lmo2 gene encodes a transcriptional cofactor critical for the development of hematopoietic stem cells. Ectopic LMO2 expression causes leukemia in T-cell acute lymphoblastic leukemia (T-ALL) patients and severe combined immunodeficiency patients undergoing retroviral gene therapy. Tightly controlled Lmo2 expression is therefore essential, yet no comprehensive analysis of Lmo2 regulation has been published so far. By comparative genomics, we identified 17 highly conserved noncoding elements, 9 ...[more]

PMID: 19171877

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