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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human primary breast cancer panel (Nottingham)

ABSTRACT: Total RNA from 128 primary breast tumors was labelled using the Illumina TotalPrep RNA Amplification kit (Ambion) following manufacturers instructions. 1.5 ug of biotin-labelled cRNA were used for each hybridisation on Sentrix Human-6 BeadChips v1.0 (Illumina, San Diego, CA) following manufacturers protocol.

ORGANISM(S): Homo sapiens

SUBMITTER: Inmaculada Spiteri

PROVIDER: E-TABM-576 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of human breast cell lines and one normal RNA sample

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2007-11-17 | E-TABM-194 | biostudies-arrayexpress

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2011-11-11 | E-TABM-827 | biostudies-arrayexpress

Transcriptional profiling in skin of K14Sin3KO mice

Project description:Expression analysis of K14Sin3afl/flCreER,K14Mycfl/flCreER and K14Sin3aMycfl/flCreER effects on gene expression in adult mouse skin. We used a conditional knock out strategy to delete Sin3a and/or c-Myc specifically in adult mouse K14 positive keratinocytes and analysed the resulting changes in gene expression by means of microarrays.

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Transcription profiling of liver tissue from humans, wild type mice and a Down syndrome mouse model containing human chromosome 21 to determine whether genetic sequence or nuclear enviornment directs tissue-specific transcription factor binding

Project description:Homologous sets of transcription factors direct conserved tissue-specific transcription, yet transcription factor binding events diverge rapidly between closely related species. We used hepatocytes from a Down syndrome mouse model containing human chromosome 21 (TC1) to determine whether human genetic sequence or mouse nuclear environment primarily determines tissue-specific transcriptional regulation. Virtually all transcription factor binding locations, transcription initiation events and the resulting gene expression observed in human hepatocytes are recapitulated across the entire human chromosome 21 in the mouse nucleus. Thus, in homologous tissues, genetic sequence is largely responsible for directing transcriptional programs, and interspecies differences in epigenetics, cellular environment, and transcription factors themselves play secondary roles.

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Project description:Transcriptional and polysomal analysis of mice liver hepatocytes after 4-hour refeeding as compared to overnight starved controls.

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Project description:Administration of low-dose IL-2 prevents and cures type 1 diabetes (T1D) in NOD mice by boosting pancreatic regulatory T cells (Tregs) and is currently being evaluated in humans. Here, to improve treatment efficacy we tested higher IL-2 doses that, despite further boosting Tregs, rapidly precipitated T1D in pre-diabetic mice due to generalized immune activation. Although the combination of rapamycin (RAPA) plus IL-2 prevents NOD T1D development, a recent clinical trial translating this strategy into patients was halted due to C-peptide decline. Here, we show that RAPA/IL-2 combination was also ineffective to cure new onset T1D in mice and, surprisingly, RAPA broke IL-2-induced tolerance in a reversible way. RAPA partially counteracted IL-2 effects on Tregs and the combined treatment unexpectedly, impaired glucose homeostasis at multiple levels, possibly explaining the clinical outcome. Our data help understand IL-2 alone or RAPA/IL-2 combination limitations and could lead to the design of improved T1D therapies.

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