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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse developing forebrain primary neuronal cultures

ABSTRACT: C57BL/6 mice at 17.5 days of pregnancy were sacrifced and forebrains of embryos were dissected. Cell plating and culture manipulation were done as previously described [E-MEXP-878]. At day 1, day 2, day 4 and day 8 of in vitro culture development, total RNA was extracted and profiled on Illumina Sentrix BeadChip Array Mouse-WG6 v1.1 microarray platform.

ORGANISM(S): Mus musculus

SUBMITTER: Sergei Manakov

PROVIDER: E-TABM-615 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Reciprocal regulation of microRNA and mRNA profiles in neuronal development and synapse formation.

Manakov Sergei A SA Grant Seth G N SG Enright Anton J AJ

BMC genomics 20090908

<h4>Background</h4>Synapse formation and the development of neural networks are known to be controlled by a coordinated program of mRNA synthesis. microRNAs are now recognized to be important regulators of mRNA translation and stability in a wide variety of organisms. While specific microRNAs are known to be involved in neural development, the extent to which global microRNA and mRNA profiles are coordinately regulated in neural development is unknown.<h4>Results</h4>We examined mouse primary ne ...[more]

PMID: 19737397

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MicroRNA profiling of mouse developing forebrain primary neuronal cultures

Project description:C57BL/6 mice at 17.5 days of pregnancy were sacrifced and forebrains of embryos were dissected. Cell plating and culture manipulation were done as previously described [E-MEXP-878]. At day 1, day 2, day 4 and day8 of in vitro culture development, total RNA was extracted and miRNAs were profiled on Illumina Sentrix Universal-96 Array Matrix with microRNA Assay Pool designed against mirBase version 9.1.

2009-09-01 | E-TABM-618 | biostudies-arrayexpress

MicroRNA profiling of mouse differentiating embryonic stem cells

Project description:To characterise changes in microRNA expression in differentiating mouse ES cells

2009-08-15 | E-TABM-621 | biostudies-arrayexpress

Retinal gene expression of low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice

Project description:Retinal gene expression was assessed from postnatal day (P) 8 old low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice with a Illumina mouse-WG6 expression BeadChip. RNA was extracted from the retinas of P8 Lrp5 knockout and wild type mice (n=3 each group). Total RNA (1ug each) were reverse transcribed, followed by a single in vitro transcription amplification to incorporate biotin-labeled nucleotide, and subsequent hybridization and staining with strepatavidin-Cy3 according to the manufacturer’s instructions.

2012-05-21 | E-GEOD-32145 | biostudies-arrayexpress

Transcription profiling of differentiating mouse embryonic stem cells

Project description:To characterize messenger RNA expression in differentiating mouse ES cells

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Retinal gene expression of low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice

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Genome-wide miRNA turnover profile in various cell types after transcription inhibition

Project description:We measured miRNA turnover rates in 8 mammalian cell types post transcription inhibitors ActinomycinD (ActD) or 5,6-Dichlorobenzimidazole 1-M-NM-2-D-ribofuranoside (DRB) treatment with miRNA expression profiling, generating 218 expression profiles on 528 endogenous miRNAs. Cells were plated in six-well plates at around 200,000 cells/well. A media change was performed one day after plating. ActD and DRB treatment were started after confluency (around two days after plating) for 0, 2, 4, 6, 12, and 24 hours post treatment with biological duplicate (ActD treatment) or biological triplicate (DRB treatment). The concentration of ActD and DRB for different cell lines were titrated to achieve rapid c-myc down-regulation after 2 hours of ActD and DRB treatment. After treatment at indicated time points, cells were washed with PBS once and lysed in TriZol reagent immediately (Invitrogen). RNA extraction was performed following the manufacturerM-bM-^@M-^Ys protocol. miRNA profiling was performed using a Luminex bead-based method.

2015-02-05 | E-GEOD-46877 | biostudies-arrayexpress

Keystone genes for tooth development [Rat RNA-Seq]

Project description:The expression levels of developing rat molar transcriptome were measured. 5 samples each from embryonic day 15.5, and 17.5 of rat developing molar tooth.

2020-11-01 | GSE158697 | GEO

Project description:Halanaerobium congolense WG6

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Impact of Maternal Immune Activation and Sex on Placental and Fetal Brain Cytokine and Gene Expression Profiles in a Preclinical Model of Neurodevelopmental Disorders

Project description:Maternal inflammation during gestation is associated with a later diagnosis of neurodevelopmental disorders including autism spectrum disorder (ASD). However, the specific impact of maternal immune activation (MIA) on placental and fetal brain development remains insufficiently understood. This study aimed to investigate the effects of MIA by analyzing placental and brain tissues obtained from the offspring of pregnant dams exposed to polyinosinic:polycytidylic acid (Poly I:C) on embryonic day 12.5. Cytokine and mRNA content in the placenta and brain tissues were assessed using multiplex cytokine assays and bulk-RNA sequencing on embryonic day 17.5. Bulk-RNA sequencing was performed on placenta and brain tissues on embryonic day 17.5.

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The BET inhibitor JQ1 selectively impairs the tumour response to hypoxia and downregulates CA9 and angiogenesis in triple receptor negative breast cancer

Project description:This study aimed to investigate whether the BET inhibitor JQ1 could alter the hypoxia-induced upregulation of gene expression and have an anti-tumour effect associated with this mechanism. We showed JQ1 downregulates 44% of hypoxia upregulated genes, including CA9 and VEGF-A. We demonstrated that JQ1 reduces triple receptor negative breast cancer (TNBC) tumour growth in monolayer and spheroid (3D) cell culture.

2016-03-31 | E-MTAB-4604 | biostudies-arrayexpress

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